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throughput sequencing results  (Biotechnology Information)

 
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    Biotechnology Information throughput sequencing results
    Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on <t>a</t> <t>high-throughput</t> platform, and bioinformatic analysis identifies translocated chromosomes
    Throughput Sequencing Results, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/throughput sequencing results/product/Biotechnology Information
    Average 86 stars, based on 1 article reviews
    throughput sequencing results - by Bioz Stars, 2026-06
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    1) Product Images from "Identification of a complex chromosomal insertion using the chromosome conformation based karyotyping technique for the implementation of PGT-SR"

    Article Title: Identification of a complex chromosomal insertion using the chromosome conformation based karyotyping technique for the implementation of PGT-SR

    Journal: BMC Genomics

    doi: 10.1186/s12864-025-12515-8

    Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on a high-throughput platform, and bioinformatic analysis identifies translocated chromosomes
    Figure Legend Snippet: Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on a high-throughput platform, and bioinformatic analysis identifies translocated chromosomes

    Techniques Used: Isolation, End Labeling, Ligation, Purification, Adapter Ligation, Amplification, High Throughput Screening Assay



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    Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on <t>a</t> <t>high-throughput</t> platform, and bioinformatic analysis identifies translocated chromosomes
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    Average 86 stars, based on 1 article reviews
    throughput sequencing results - by Bioz Stars, 2026-06
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    Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on <t>a</t> <t>high-throughput</t> platform, and bioinformatic analysis identifies translocated chromosomes
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    Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on <t>a</t> <t>high-throughput</t> platform, and bioinformatic analysis identifies translocated chromosomes
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    Image Search Results


    Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on a high-throughput platform, and bioinformatic analysis identifies translocated chromosomes

    Journal: BMC Genomics

    Article Title: Identification of a complex chromosomal insertion using the chromosome conformation based karyotyping technique for the implementation of PGT-SR

    doi: 10.1186/s12864-025-12515-8

    Figure Lengend Snippet: Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on a high-throughput platform, and bioinformatic analysis identifies translocated chromosomes

    Article Snippet: The high-throughput sequencing results of 20 biopsies of human early blastocyst trophoblast cells analyzed during this study are publicly available in the National Center for Biotechnology Information BioSample database under accession number PRJNA128600 ( https://www.ncbi.nlm.nih.gov/sra/PRJNA1286004 ).

    Techniques: Isolation, End Labeling, Ligation, Purification, Adapter Ligation, Amplification, High Throughput Screening Assay